Abstract

Seed oil extract of Hildegardia barteri was investigated for antimicrobial activities against some bacteria and fungi species using the microbial inhibition assay.  An aliquot (0.1ml) of each seed oil extract was tested for anti-bacteria and anti-fungi activity. Fluconazole and Streptomycin were used as control for fungi (Aspergillus niger, Trichophyton species and Candidas albicans) and bacteria (E. coli, S. aureus, P. aeruginosa and B. subtilis) respectively. The results showed that there was no antifungal activity against Aspergillus niger, Trichophyton spp and Candidas albicans. The antifungal sensitivity test of seed oil extract of Hildagardia barteri using agar well diffusion assay showed that there were no zones of inhibition on the growth against C. albicans and Trichophyton sp. After 48 hours of incubation. Similarly there was no growth inhibitory activity when the positive control (Fluconazole) was used. However, there was growth inhibitory activity against Aspergillus niger. There was also no anti-bacterial activity when seed oil extract of Hildegardia barteri was used against E. coli, S. aureus, P. aeruginosa and B. subtilis for the zones of inhibition were 0.0mm.  However, the zones of inhibition after 24hours of incubation using Streptomycin as the control showed that E. coli was 270mm, S. aureus was 390mm, P. aeruginosa was 365mm and B. subtilis was 385mm. Thus, the research results suggested that the seed oil extract of Hildergadia barteri did not provide antimicrobial properties against the fungi and bacteria used in this study